Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions
Electron. j. biotechnol
;
19(6): 12-20, Nov. 2016. ilus
Article
in English
| LILACS
| ID: biblio-840307
ABSTRACT
Background:
Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated.Results:
Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing statistical method based on the central composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264, which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and Mg2+ at 0.13 mmol/L in medium.Conclusion:
The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an effective strategy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ascomycota
/
Pyrones
/
Heterocyclic Compounds, 3-Ring
Type of study:
Prognostic study
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2016
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
China Agricultural University/CN
/
Ministry of Agriculture/CN
Similar
MEDLINE
...
LILACS
LIS