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Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions
Luo, Haiyu; Xu, Dan; Xie, Rushan; Zhang, Xuping; Wang, Jian; Xuejiao, Dong; Lai, Daowan; Zhou, Ligang; Liu, Yang.
Affiliation
  • Luo, Haiyu; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Xu, Dan; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Xie, Rushan; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Zhang, Xuping; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Wang, Jian; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Xuejiao, Dong; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Lai, Daowan; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Zhou, Ligang; China Agricultural University. College of Plant Protection. Ministry of Agriculture/Department of Plant Pathology. Key Laboratory of Plant Pathology. Beijing. CN
  • Liu, Yang; Ministry of Agriculture. College of Plant Protection. Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products. Beijing. CN
Electron. j. biotechnol ; Electron. j. biotechnol;19(6): 12-20, Nov. 2016. ilus
Article in En | LILACS | ID: biblio-840307
Responsible library: CL1.1
ABSTRACT

Background:

Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated.

Results:

Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing statistical method based on the central composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264, which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and Mg2+ at 0.13 mmol/L in medium.

Conclusion:

The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an effective strategy.
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Full text: 1 Index: LILACS Main subject: Ascomycota / Pyrones / Heterocyclic Compounds, 3-Ring Type of study: Prognostic_studies Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2016 Type: Article / Project document

Full text: 1 Index: LILACS Main subject: Ascomycota / Pyrones / Heterocyclic Compounds, 3-Ring Type of study: Prognostic_studies Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2016 Type: Article / Project document