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Antiangiogenic VEGF165b expression in human breast MCF-7 and MCF-10A cells exposed to reverse transcriptase and protease inhibitors / Expresión de VEGF165b antiangiogénico en células MCF-7 y MCF-10A de mama humana expuesto a inhibidores de la transcriptasa inversa y la proteasa
Adefolaju, Gbenga Anthony; Scholtz, Kathrine Elizabeth; Hosie, Margot Jill.
  • Adefolaju, Gbenga Anthony; University of Limpopo Private. School of Health Care Sciences. Department of Preclinical Sciences. Sovenga. ZA
  • Scholtz, Kathrine Elizabeth; University of Limpopo Private. School of Health Care Sciences. Department of Preclinical Sciences. Sovenga. ZA
  • Hosie, Margot Jill; University of the Witwatersrand. School of Anatomical Sciences. ZA
Int. j. morphol ; 35(1): 148-156, Mar. 2017. ilus
Article in English | LILACS | ID: biblio-840946
ABSTRACT
The combined antiretroviral therapy (cART), a multidrug combination regimen, usually consisting Nucleoside Reverse Transcriptase Inhibitors, non- Nucleoside Reverse Transcriptase Inhibitors and Protease Inhibitors has altered the morbidity pattern affecting HIV-infected individuals to include non-AIDS-defining malignancies (nADMs). The speculation is rife; does cART induce or promote the progression of nADMs such as breast cancer? This study was therefore designed to investigate of the effects of some antiretroviral drugs (at clinically relevant concentrations) on the expression of anti-angiogenic gene; VEGF165b in two human breast cell lines; MCF-7 and MCF-10A by Real Time qPCR and immuno-fluorescence. All of the antiretroviral drugs and combinations tested produced patterns of slight up or downregulation of VEGF165b mRNA expression but the alterations did not attain statistical significance. They also did not alter VEGF165bprotein localisation in both cell lines. The findings reported here suggest that antiretroviral drugs probably do not influence the angiogenic pathway in the development of breast cancer in patients under the combined antiretroviral regimen.
RESUMEN
El tratamiento antirretroviral combinado (TARc), un régimen de combinación de múltiples fármacos, consistiendo generalmente en inhibidores nucleósidos de la transcriptasa reversa, inhibidores no-nucleósidos de la transcriptasa reversa e inhibodres de proteasa que alteran el patrón de mortalidad que afecta a infectados por el VIH incluyendo neoplasias definidas como no HIV (nADMs). La especulación es moneda corriente; TARc induce o promueve la progresión de nADMs como cáncer de mama? Por lo tanto, este estudio se diseñó para investigar los efectos de algunos de los fármacos antirretrovirales (en concentraciones clínicamente relevantes) sobre la expresión del gen anti-angiogénico; VEGF165b en dos líneas celulares de mama humana; MCF-7 y MCF-10A por PCR tiempo real e inmunofluorescencia. Todos los fármacos antirretrovirales y las combinaciones probadas pueden regular en forma ligera hacia arriba o hacia abajo la expresión de ARNm producidos por VEGF165b pero las alteraciones no fueron estadísticamente significativos. Además, no se alteran los niveles de proteína VEGF165b, para la localización en ambas líneas celulares. Los resultados aquí presentados sugieren que los medicamentos antirretrovirales probablemente no influyen en la vía angiogénica en el desarrollo del cáncer de mama en pacientes bajo el régimen antirretroviral combinado.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Protease Inhibitors / Breast Neoplasms / Adenocarcinoma / Reverse Transcriptase Inhibitors / Angiogenesis Inhibitors Limits: Female / Humans Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2017 Type: Article Affiliation country: South Africa Institution/Affiliation country: University of Limpopo Private/ZA / University of the Witwatersrand/ZA

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Full text: Available Index: LILACS (Americas) Main subject: Protease Inhibitors / Breast Neoplasms / Adenocarcinoma / Reverse Transcriptase Inhibitors / Angiogenesis Inhibitors Limits: Female / Humans Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2017 Type: Article Affiliation country: South Africa Institution/Affiliation country: University of Limpopo Private/ZA / University of the Witwatersrand/ZA