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Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses
NOGALES, Carlos Goes; FERREIRA, Marina Beloti; MONTEMOR, Antonio Fernando; RODRIGUES, Maria Filomena de Andrade; Lage-MARQUES, José Luiz; ANTONIAZZI, João Humberto.
  • NOGALES, Carlos Goes; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
  • FERREIRA, Marina Beloti; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
  • MONTEMOR, Antonio Fernando; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
  • RODRIGUES, Maria Filomena de Andrade; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
  • Lage-MARQUES, José Luiz; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
  • ANTONIAZZI, João Humberto; Universidade de São Paulo. Faculdade de Odontologia. Departamento de Endodontia. São Paulo. BR
J. appl. oral sci ; 24(6): 607-613, Nov.-Dec. 2016. tab
Article in English | LILACS, BBO | ID: biblio-841151
ABSTRACT
ABSTRACT Objectives This study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus using a mono-species biofilm model. Parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. Material and

Methods:

One hundred and eighty single-root teeth were contaminated with a mono-species biofilm of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Groups were formed Group I – control; Group II – standard protocol; Group III – standard protocol + ozone gas at 40 µg/mL; and Group IV – standard protocol + aqueous ozone at 8 µg/mL. In parallel, human gingival fibroblasts were submitted to the MTT test. Cells were plated, then ozone was applied as follows Group I (control) – broth medium; Group II – aqueous ozone at 2 µg/mL; Group III – aqueous ozone at 5 µg/mL; and Group IV – aqueous ozone at 8 µg/mL. Data were submitted to the Kruskal Wallis test and Bonferroni post hoc analyses to assess microbiology and cytotoxicity, respectively (p<0.05%). Results The results revealed antimicrobial efficacy by Group IV with no CFU count. The cytotoxicity assay showed Groups III and IV to be the most aggressive, providing a decrease in cell viability at hour 0 from 100% to 77.3% and 68.6%, respectively. Such a decrease in cell viability was reverted, and after 72 hours Groups III and IV provided the greatest increase in cell viability, being statistically different from Groups I and II. Conclusion According to the applied methodology and the limitations of this study, it was possible to conclude that ozone therapy improved the decontamination of the root canal ex vivo. Ozone was toxic to the cells on first contact, but cell viability was recovered. Thus, these findings suggest that ozone might be useful to improve root canal results.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ozone / Pseudomonas aeruginosa / Staphylococcus aureus / Enterococcus faecalis / Dental Pulp Cavity / Fibroblasts / Anti-Infective Agents Type of study: Evaluation studies / Practice guideline / Prognostic study Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ozone / Pseudomonas aeruginosa / Staphylococcus aureus / Enterococcus faecalis / Dental Pulp Cavity / Fibroblasts / Anti-Infective Agents Type of study: Evaluation studies / Practice guideline / Prognostic study Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR