Validated high performance thin layer chromatography method for simultaneous determination of quercetin and gallic acid in Leea indica
Rev. bras. farmacogn
;
27(1): 50-53, Jan.-Feb. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-843789
ABSTRACT
ABSTRACT A sensitive and reliable high performance thin layer chromatography method has been developed for the simultaneous estimation of quercetin and gallic acid in Leea indica, Vitaceae. Ethyl acetate extract prepared from hydrolysed aqueous alcoholic extract (70%) was applied on silica gel G 60 F254 plate. The plate was developed using toluene-ethyl acetate-formic acid, 541 (v/v/v) as a mobile phase and detection and quantification were performed by densitometric scanning at 254 nm. The system was found to give well resolved bands for quercetin (Rf 0.63) and gallic acid (Rf 0.45) from other constituents present in the extract of L. indica. The correlation coefficient was found to be 0.991 and 0.999 with relative standard deviation, 0.97–1.23% and 0.1–1.13% for quercetin and gallic acid respectively in the developed method. The accuracy of the method was confirmed by conducting recovery studies at different levels using the standard addition method. The average recovery of quercetin and gallic acid was found close to 99% suggesting the accurateness of the method. The proposed validated high performance thin layer chromatographic method offers a new, sensitive, specific and precise gauge for quantification of quercetin and gallic acid in L. indica.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. bras. farmacogn
Journal subject:
Pharmacy
Year:
2017
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
L. M. College of Pharmacy/IN
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