Collagenous matrix as a predictor for bone formation: a digital technique for collagen quantification
RFO UPF
;
22(1): 18-24, 28/08/2017.
Article
in English
| LILACS
| ID: biblio-848698
ABSTRACT
The collagenous matrix plays a fundamental role in the process of bone regeneration, so it is essential to study how it is primarily formed in situations in which critical bone defects are created. Objective: this study seeks to quantify the collagenous matrix formed in critical bone defects in the calvaria of mice over the process of bone regeneration promoted by the association of poly(lactide-co-glycolide) (PLGA) porous scaffolds and stem cells from deciduous teeth (SCDT). In addition, this study attempted to establish a precise protocol for the digital quantification of collagen through a histological method. Materials and method: Nine Wistar rats were used, in which critical defects of 8.0 mm of diameter were made in their calvarium. The animals were divided into three groups (n = 9): I PLGA scaffolds; II PLGA scaffolds/SCDT; III PLGA scaffolds/SCDT maintained in osteogenic medium for 13 days. Within sixty postoperative days, calvaria were removed for histometric analysis following a digital protocol. A specific digital analysis method was designed for this study, in which a more precise quantification and differentiation between collagen fibers and non-collagenous tissue was possible, excluding factors that would normally alter the results. Results: it was noted that the association of PLGA scaffolds and SCDT maintained in osteogenic medium resulted in collagen matrix formation statistically higher than the other groups (p<0.05). Conclusion: the protocol designed for collagen quantification was precise and efficient, producing methodologically standardized results.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Prognostic study
/
Risk factors
Language:
English
Journal:
RFO UPF
Journal subject:
Dentistry
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UFRGS/BR
/
Universidade de São Paulo/BR
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