Evaluation of coronal microleakage: bacterial versus dye infiltration tests

ABSTRACT

The aim of this study was to verify, in vitro, two methodologies of coronal microleakage after endodontic access cavity filling with temporary restorative material. Twenty-four extracted single rooted teeth were selected, of which four specimens were used for methodology control. Standard access cavities were prepared in 20 teeth, followed by root canal instrumentation and coronal access sealing with temporary restorative material (Villevie-Dentalville, Joinville, Santa Catarina, Brazil). The teeth were externally waterproofed and submitted to infiltration tests. Fourteen samples were used for microbiology infiltration analysis with Enterococcus faecalis. The other 10 samples were used for dye leakage analysis with 1% rhodamine B tracer. The results demonstrated no infiltration of Enterococcus faecalis in any of the samples at the end of 30 days. However, dye leakage analysis showed infiltration through the temporary restoration in all samples (mean of infiltration = 84.3%). Significant statistical differences (p < 0.05) were found between the two methodologies. In conclusion, bacterial and dye leakage methodologies showed contradictory results and the type of performed methodology can significantly influence the results of coronal microleakage analysis: