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Tissue expresion of the genes mutyh and ogg1 in patients with sporadic colorectal cancer / Expressão tecidual dos genes mutyh e ogg1 em doentes com câncer colorretal esporádico
Nascimento, Enzo Fabrício Ribeiro; Ribeiro, Marcelo Lima; Magro, Daniela Oliveira; Carvalho, Juliana; Kanno, Danilo Toshio; Martinez, Carlos Augusto Real; Coy, Cláudio Saddy Rodrigues.
  • Nascimento, Enzo Fabrício Ribeiro; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Ribeiro, Marcelo Lima; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Magro, Daniela Oliveira; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Carvalho, Juliana; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Kanno, Danilo Toshio; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Martinez, Carlos Augusto Real; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
  • Coy, Cláudio Saddy Rodrigues; State University of Campinas. Faculty of Medical Sciences. Postgraduate Program in Surgery Sciences. Campinas. BR
ABCD (São Paulo, Impr.) ; 30(2): 98-102, Apr.-June 2017. tab
Article in English | LILACS | ID: biblio-885711
ABSTRACT
ABSTRACT

Background:

MTUYH and OGG1 genes have importance in the base excision repair systems of oxidized DNA bases. Modification of the tissue expression of these genes is related to the increased risk of developing colorectal cancer.

Aim:

To evaluate the tissue expression of MUTYH and OGG1 comparing normal and neoplastic tissues of patients with sporadic colorectal cancer and to correlate it with clinical and histopathological variables.

Method:

MUTYH and OGG1 tissue expression was quantified by RT-PCR in patients with colorectal cancer and the values were compared in normal and neoplastic tissues. MUTYH and OGG1 expression was measured and normalized to the constitutive 18S gene. The level of expression of both genes was correlated with the variables age, gender, tumor location, size of the tumor, histological type, degree of cell differentiation, invasion depth in the intestinal wall, angiolymphatic infiltration, lymph node involvement and TNM staging.

Results:

Was found downregulation of both genes in neoplastic when compared to normal tissue. There was downregulation of the MUTYH in larger tumors and in patients with angiolymphatic invasion. Tumors with more advanced TNM stages (III and IV) presented downregulation of both genes when compared to those with earlier stages (I and II).

Conclusion:

The MUTYH and OGG1 genes present downregulation in the more advanced stages of colorectal cancer.
RESUMO
RESUMO Racional Os genes MUTYH e OGG1 possuem importância nos sistemas de reparo por excisão de bases oxidadas do DNA. Modificação na expressão tecidual desses genes encontra-se relacionada ao maior risco do desenvolvimento do câncer colorretal.

Objetivo:

Avaliar a expressão tecidual dos genes MUTYH e OGG1 comparando tecidos normais e neoplásicos de portadores de câncer colorretal esporádico e correlacioná-la com variáveis clínicas e histopatológicas.

Método:

Avaliou-se por PCR, em tempo real, a expressão tecidual dos genes MUTYH e OGG1 em 49 portadores de câncer colorretal comparando tecidos normais e neoplásicos. A expressão dos genes MUTYH e OGG1 foi quantificada e normalizada com o gene constitutivo 18S. A intensidade de expressão de ambos os genes foi correlacionada as variáveis idade, gênero, localização do tumor, tamanho do tumor, tipo histológico, grau de diferenciação celular, profundidade de invasão na parede intestinal, invasão angiolinfática, linfonodos comprometidos e estadiamento TNM.

Resultados:

Encontrou-se menor expressão de ambos os genes no tecido neoplásico quando comparado ao tecido normal. Houve menor expressão do gene MUTYH nos tumores com maiores dimensões e nos doentes que apresentavam invasão angiolinfática. Tumores com estadios mais avançados (III e IV) apresentavam expressão menor de ambos os genes quando comparados àqueles com estadios mais precoces (I e II).

Conclusão:

Os genes MUTYH e OGG1 apresentam menor expressão tecidual nos estadios mais avançados do câncer colorretal.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Colorectal Neoplasms / Adenocarcinoma / Gene Expression Regulation, Neoplastic / DNA Glycosylases Type of study: Observational study / Risk factors Limits: Adult / Aged / Female / Humans / Male Language: English Journal: ABCD (São Paulo, Impr.) Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR

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Full text: Available Index: LILACS (Americas) Main subject: Colorectal Neoplasms / Adenocarcinoma / Gene Expression Regulation, Neoplastic / DNA Glycosylases Type of study: Observational study / Risk factors Limits: Adult / Aged / Female / Humans / Male Language: English Journal: ABCD (São Paulo, Impr.) Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR