Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs
Acta cir. bras
;
32(11): 891-902, Nov. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-886185
ABSTRACT
Abstract Purpose:
To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments.Methods:
Three mini-pigs(Minipig-BR) with age of 8 months (weight 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP.Results:
The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed.Conclusion:
The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Transplantation, Autologous
/
Adipose Tissue
/
Plastic Surgery Procedures
/
Disease Models, Animal
Type of study:
Prognostic study
/
Qualitative research
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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