Biomechanic and histologic analysis of fibroblastic effects of tendon to bone healing by transforming growth factor ß1 (TGF-ß1) in rotator cuff tears
Acta cir. bras
;
32(12): 1045-1055, Dec. 2017. graf
Article
in English
| LILACS
| ID: biblio-886197
ABSTRACT
Abstract Purpose:
To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears.Methods:
Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method.Results:
Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages.Conclusion:
The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β1.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tendon Injuries
/
Wound Healing
/
Rotator Cuff
/
Vascular Endothelial Growth Factors
/
Transforming Growth Factor beta1
/
Rotator Cuff Injuries
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2017
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Chinese PLA General Hospital, Beijing/CN
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