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Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study
Roque, José Sidney; Pomini, Karina Torres; Buchaim, Rogério Leone; Buchaim, Daniela Vieira; Andreo, Jesus Carlos; Roque, Domingos Donizeti; Rodrigues, Antonio de Castro; Rosa Júnior, Geraldo Marco; Moraes, Luis Henrique Rapucci; Viterbo, Fausto.
  • Roque, José Sidney; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Pomini, Karina Torres; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Buchaim, Rogério Leone; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Buchaim, Daniela Vieira; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Andreo, Jesus Carlos; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Roque, Domingos Donizeti; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Rodrigues, Antonio de Castro; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Rosa Júnior, Geraldo Marco; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Moraes, Luis Henrique Rapucci; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
  • Viterbo, Fausto; Universidade Estadual do Norte do Paraná. Department of Anatomy. Jacarezinho. BR
Acta cir. bras ; 32(8): 617-625, Aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-886230
ABSTRACT
Abstract

Purpose:

To evaluated the tubulization technique with standard and inside-out vein, filled or not with platelet-rich plasma (PRP), in sciatic nerve repair.

Methods:

Seventy male Wistar rats were randomly divided into five groups IOVNF (Inside-Out Vein with No Filling); IOVPRP (Inside-Out Vein filled with PRP); SVNF (Standard Vein with No Filling); SVPRP (Standard Vein filled with PRP); Sham (Control). The left external jugular vein was used as graft in a 10 mm nervous gap.

Results:

In the morphological analysis of all groups, myelinated nerve fibers with evident myelin sheath, neoformation of the epineurium and perineurium, organization of intraneural fascicles and blood vessels were observed. In the morphometry of the distal stump fibers, SVPRP group had the highest means regarding fiber diameter (3.63±0.42 μm), axon diameter (2.37±0.31 μm) and myelin sheath area (11.70±0.84 μm2). IOVPRP group had the highest means regarding axon area (4.39±1.16 μm2) and myelin sheath thickness (0.80±0.19 μm). As for values of the fiber area, IOVNF group shows highest means (15.54±0.67 μm2), but are still lower than the values of the Sham group.

Conclusion:

The graft filled with platelet-rich plasma, with use standard (SVPRP) or inside-out vein (IOVPRP), promoted the improvement in axonal regeneration on sciatic nerve injury.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Sciatic Nerve / Guided Tissue Regeneration / Platelet-Rich Plasma / Jugular Veins / Myelin Sheath / Nerve Regeneration Type of study: Evaluation studies / Prognostic study / Risk factors Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Norte do Paraná/BR

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Full text: Available Index: LILACS (Americas) Main subject: Sciatic Nerve / Guided Tissue Regeneration / Platelet-Rich Plasma / Jugular Veins / Myelin Sheath / Nerve Regeneration Type of study: Evaluation studies / Prognostic study / Risk factors Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Norte do Paraná/BR