Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
Acta cir. bras
;
33(2): 144-155, Feb. 2018. graf
Article
in English
| LILACS
| ID: biblio-886256
ABSTRACT
Abstract Purpose:
To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.Methods:
Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18) group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.Results:
The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).Conclusion:
Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Skin
/
Wound Healing
/
Biological Dressings
/
Collagen
/
Amnion
Type of study:
Controlled clinical trial
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade do Vale do Paraíba/BR
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