Your browser doesn't support javascript.
loading
Efficacy of phosphocreatine pre-administration on XIAP and Smac in ischemic penumbra of rats with focal cerebral ischemia reperfusion injury
Wang, Wei; Wang, Qi; Yu, Wanyou; Chen, Lianhua; Li, Zhong.
  • Wang, Wei; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. CN
  • Wang, Qi; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. CN
  • Yu, Wanyou; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. CN
  • Chen, Lianhua; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. CN
  • Li, Zhong; Nanjing Medical University. Jiangning Hospital Affiliated. Department of Anesthesiology. CN
Acta cir. bras ; 33(2): 117-124, Feb. 2018. tab, graf
Article in English | LILACS | ID: biblio-886260
ABSTRACT
Abstract

Purpose:

To observe the efficacy of phosphocreatine pre-administration (PCr-PA) on X-linked inhibitor of apoptosis protein (XIAP), the second mitochondia-derived activator of caspase (Smac) and apoptosis in the ischemic penumbra of rats with focal cerebral ischemia-reperfusion injury (CIRI).

Methods:

A total of 60 healthy male Sprague Dawley (SD) rats were randomly divided into three groups (n=20) group A (the sham operation group), group B <intraperitoneally injected with 20 mg/kg (10 mg/ml) of saline before preparing the ischemia-reperfusion (IR) model>, and group C <intraperitoneally injected with 20 mg/kg (10 mg/ml) of PCr immediately before preparing the IR model>. After 24 h for reperfusion, the neurological function was evaluated and the tissue was sampled to detect expression of XIAP, Smac and caspase-3 positive cells in the ischemic penumbra so as to observe the apoptosis.

Results:

Compared with group B, neurological deficit scores, numbers of apoptotic cells, expression of Smac,caspase-9 and the numbers of Caspase-3 positive cells were decreased while expression of XIAP were increased in the ischemic penumbra of group C.

Conclusions:

Phosphocreatine pre-administration may elicit neuroprotective effects in the brain by increasing expression of X-linked inhibitor of apoptosis protein, reducing expression of second mitochondia-derived activator of caspase, and inhibiting the apoptosis in the ischemic penumbra.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Phosphocreatine / Cardiotonic Agents / Reperfusion Injury / Brain Ischemia / Mitochondrial Proteins / Intracellular Signaling Peptides and Proteins / X-Linked Inhibitor of Apoptosis Protein Type of study: Controlled clinical trial / Prognostic study Limits: Animals / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Nanjing Medical University/CN

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Phosphocreatine / Cardiotonic Agents / Reperfusion Injury / Brain Ischemia / Mitochondrial Proteins / Intracellular Signaling Peptides and Proteins / X-Linked Inhibitor of Apoptosis Protein Type of study: Controlled clinical trial / Prognostic study Limits: Animals / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Nanjing Medical University/CN