Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei, Mytilidae) in environmental samples
An. acad. bras. ciênc
;
89(2): 1041-1045, Apr.-June 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-886687
ABSTRACT
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mytilidae
/
Introduced Species
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
An. acad. bras. ciênc
Journal subject:
Science
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Paraná/BR
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