Cryopreservation of banana's cv Grand Naine in vitro rhizomes
An. acad. bras. ciênc
;
90(1): 495-507, Mar. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-886920
ABSTRACT
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Phloroglucinol
/
Cryopreservation
/
Cryoprotective Agents
/
Musa
/
Rhizome
Type of study:
Evaluation studies
Language:
English
Journal:
An. acad. bras. ciênc
Journal subject:
Science
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Empresa de Pesquisa Agropecuária de Minas Gerais/BR
Similar
MEDLINE
...
LILACS
LIS