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Effects of drying and storage conditions on the stability of TSH in blood spots
Magalhães, Patrícia Künzle Ribeiro; Miranda, Carlos Henrique; Vilar, Fernando Crivelenti; Schmidt, André; Bittar, Roberta Rodrigues; Paixão, Giselle Aparecida Caixe de Carvalho; Martinez, Edson Zangiacomi; Maciel, Léa Maria Zanini.
Affiliation
  • Magalhães, Patrícia Künzle Ribeiro; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Miranda, Carlos Henrique; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Vilar, Fernando Crivelenti; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Schmidt, André; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Bittar, Roberta Rodrigues; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Paixão, Giselle Aparecida Caixe de Carvalho; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Martinez, Edson Zangiacomi; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
  • Maciel, Léa Maria Zanini; Universidade de São Paulo. Departamento de Medicina Interna, Faculdade de Medicina de Ribeirão Preto. Divisão de Endocrinologia. Ribeirão Preto. BR
Arch. endocrinol. metab. (Online) ; 62(2): 201-204, Mar.-Apr. 2018. tab, graf
Article in En | LILACS | ID: biblio-887641
Responsible library: BR1.1
ABSTRACT
ABSTRACT Objective To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Subjects and methods Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. Results The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Conclusions Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.
Subject(s)
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Full text: 1 Index: LILACS Main subject: Thyrotropin / Blood Specimen Collection / Neonatal Screening / Freeze Drying Type of study: Evaluation_studies Limits: Adult / Aged / Female / Humans / Male / Newborn Language: En Journal: Arch. endocrinol. metab. (Online) Journal subject: ENDOCRINOLOGIA / METABOLISMO Year: 2018 Type: Article

Full text: 1 Index: LILACS Main subject: Thyrotropin / Blood Specimen Collection / Neonatal Screening / Freeze Drying Type of study: Evaluation_studies Limits: Adult / Aged / Female / Humans / Male / Newborn Language: En Journal: Arch. endocrinol. metab. (Online) Journal subject: ENDOCRINOLOGIA / METABOLISMO Year: 2018 Type: Article