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Associação à positividade da Lawsonia intracellularis com a expressão clínico-patológica da infecção em suínos da região metropolitana de Bucaramanga (Santander, Colômbia) / Association to positivity of Lawsonia intracellularis with the clinical and pathologic expression of infection in swine from the metropolitan area of Bucaramanga (Santander, Colombia)
Jiménez, A. P; Duarte, L. Z; Cortez, L. S; Granados, M; Florez, C; Villamizar, A; Parra-Martin, J. A.
  • Jiménez, A. P; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Duarte, L. Z; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Cortez, L. S; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Granados, M; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Florez, C; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Villamizar, A; Universidad Cooperativa de Colombia. Bucaramanga. CO
  • Parra-Martin, J. A; Universidad Cooperativa de Colombia. Bucaramanga. CO
Arq. bras. med. vet. zootec. (Online) ; 70(1): 181-186, Jan.-Feb. 2018. graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-888088
RESUMO
Porções de íleo terminal foram coletados de 100 suínos com sinais de doença gastrointestinal na área metropolitana de Bucaramanga, a fim de se estudar a eficiência do diagnóstico de enteropatia proliferativa suína (PPE) pela técnica de PCR aninha (PCRa) empregando sequências específicas (primers) para L. intracellularis 16S ARN região (270pb) e sua correlação com achados clínicos e patológicos. Todas as amostras foram processadas para se determinar a associação entre positividade por PCR, os sinais clínicos, os achados de necropsia e as lesões histológicas. Cinquenta e seis por cento das amostras foram positivas para L. intracellularis pela PCRa. Só 2% exibiram resultados positivos pela técnica Warthin-Starry. Trinta e um de 100 animais com sinais de anorexia resultaram positivos para PCRa (P>0,05). Não houve associação (P<0,05) entre diarreia e queda no crescimento, bem como associação (P<0,05) entre achados anatomopatológicos e histológicos com PCRa positivas.(AU)
ABSTRACT
Fragments of terminal ileum were collected from 100 pigs at slaughter from Bucaramanga Metropolitan Area (Santander, Colombia), to study the efficacy of the diagnosis of porcine proliferative enterophaty (PPE) through the technique of nested polymerase chain reaction (PCRa), employing specific sequences (primers) for L. intracellularis 16S ARN region (270pb) and his correlation with clinic and pathological findings. All samples were processed by standard histological methods and stained with a Warthin-Starry technique. All samples were processed to determinate the association between positive PCRa results, clinical signs and necropsy findings. 56% of the 100 samples were positive for L. intracellularis through PCRa technic. Only 2% exhibited positive results through Warthin-Starry technique. A total of 31 (100) animals with anorexic symptoms were associated with positive results from PCRa (P>,05). No associations (P<0.05) were observed between diarrhea and delayed growth. No associations (P<0.05) were observed between anatomopathological and histological findings with positive PCRa.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Swine / Polymerase Chain Reaction / Ileitis Type of study: Risk factors Limits: Animals Country/Region as subject: South America / Colombia Language: Portuguese Journal: Arq. bras. med. vet. zootec. (Online) Year: 2018 Type: Article Institution/Affiliation country: Universidad Cooperativa de Colombia/CO

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Full text: Available Index: LILACS (Americas) Main subject: Swine / Polymerase Chain Reaction / Ileitis Type of study: Risk factors Limits: Animals Country/Region as subject: South America / Colombia Language: Portuguese Journal: Arq. bras. med. vet. zootec. (Online) Year: 2018 Type: Article Institution/Affiliation country: Universidad Cooperativa de Colombia/CO