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The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Chen, Y; Yang, S; Peng, Y; Yang, Z.
  • Chen, Y; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Yang, S; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Peng, Y; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Yang, Z; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
Braz. j. med. biol. res ; 50(11): e6246, 2017. graf
Article in English | LILACS | ID: biblio-888947
ABSTRACT
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P<0.05). The 3′UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P<0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
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Full text: Available Index: LILACS (Americas) Main subject: Receptors, Interleukin-6 / MicroRNAs / Hepatitis B / Liver Cirrhosis Type of study: Prognostic study / Risk factors Limits: Adult / Aged / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Linyi People's Hospital/CN

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Full text: Available Index: LILACS (Americas) Main subject: Receptors, Interleukin-6 / MicroRNAs / Hepatitis B / Liver Cirrhosis Type of study: Prognostic study / Risk factors Limits: Adult / Aged / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: Linyi People's Hospital/CN