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Cordyceps sinensis promotes immune regulation and enhances bacteriostatic activity of PA-824 via IL-10 in Mycobacterium tuberculosis disease
Li, DG; Ren, ZX.
  • Li, DG; East Medical District of Linyi People's Hospital. Department of Respiratory Medicine. Linyi. CN
  • Ren, ZX; East Medical District of Linyi People's Hospital. Department of Respiratory Medicine. Linyi. CN
Braz. j. med. biol. res ; 50(9): e6188, 2017. graf
Article in English | LILACS | ID: biblio-888993
ABSTRACT
PA-824 is a novel bicyclic nitroimidazole anti-tuberculosis (TB) drug. Cordyceps sinensis (Berk.) Sacc. (CS) was proven to be a good immunomodulatory compound. This research aimed to investigate the effect of CS on PA-824 in Mycobacterium tuberculosis (M.tb) infected mice (female CBA/J mice, 6 to 8 weeks of age and 20±2 g of weight). Mice were randomly assigned to 4 groups PA-824, CS, PA-824+CS, and control. To verify the effect of PA-824 and CS on M.tb, after drug administration, mice lungs were harvested and bacterial colony formations were measured. Cells were isolated from infected lungs and spleens to analyze the percentage of CD4+ T cells (CD11a positive). Lung cells were cultured to detect the secretion of interferon-γ (IFN-γ) and interleukin-10 (IL-10) by ELISA. IFN-γ and IL-10 double-positive CD4+ cells in peripheral blood were measured by flow cytometry. The expression levels of IL-2 and IL-10 in mice lungs were analyzed by real-time PCR and western blot. Results showed that PA-824 combined with CS led to the lowest lung colony-forming units (CFU) counts among treated groups. Furthermore, this beneficial outcome might be associated with the decreased CD11a on CD4+ cells in mice lungs and spleens. Moreover, the suppressed secretion of IFN-γ and IL-10, and IL-10 expressions, as well as the decreased IFN-γ and IL-10 double-positive CD4+ cells in blood, could also be associated with the positive effect. However, no significant effect on IL-2 production was found. The combination of PA-824 and CS had more effective bacteriostatic and immunomodulatory effects on M.tb infected mice than PA-824 alone. In conclusion, CS has the potential to be an effective adjuvant in TB treatment.
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Full text: Available Index: LILACS (Americas) Main subject: Interleukin-10 / Cordyceps / Anti-Bacterial Agents / Mycobacterium tuberculosis / Nitroimidazoles Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: East Medical District of Linyi People's Hospital/CN

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Full text: Available Index: LILACS (Americas) Main subject: Interleukin-10 / Cordyceps / Anti-Bacterial Agents / Mycobacterium tuberculosis / Nitroimidazoles Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: East Medical District of Linyi People's Hospital/CN