Homology modeling and epitope prediction of Der f 33
Braz. j. med. biol. res
;
51(5): e6213, 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-889085
ABSTRACT
Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tubulin
/
Allergens
/
Epitopes, T-Lymphocyte
/
Epitopes, B-Lymphocyte
/
Dermatophagoides farinae
/
Antigens, Dermatophagoides
Type of study:
Prognostic study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2018
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Jiangsu Vocational College of Medicine/CN
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