Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
Braz. j. med. biol. res
;
51(6): e7221, 2018. tab
Article
in English
| LILACS
| ID: biblio-889096
ABSTRACT
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR. When tested against spiked samples, the previously described primers showed different sensitivity, with very similar results when samples from patients (serum and urine) were analyzed. Real-time RT-PCR designed to amplify Zika virus NS1 showed the best analytical sensitivity for all samples.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA, Viral
/
Dengue
/
Chikungunya Fever
/
Zika Virus
/
Zika Virus Infection
Type of study:
Diagnostic study
/
Practice guideline
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2018
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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