Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM)
Braz. j. microbiol
;
48(3): 427-441, July-Sept. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-889130
ABSTRACT
Abstract The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and agitation speed were slightly lower. The intra-extracellular inulinase, invertase production and biomass content increased 10-32 fold in the optimized medium condition (pH 6.5, temperature 30 °C, 6% (v/v), inoculum size and 150 rpm agitation speed) by RSM compared with medium optimized through the one-factor-at-a-time method. The process development and intensification for simultaneous production of intra-extracellular inulinase (exo and endo inulinase) and invertase from A. niger could be used for industrial applications.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aspergillus niger
/
Industrial Microbiology
/
Beta-Fructofuranosidase
/
Glycoside Hydrolases
Type of study:
Evaluation studies
/
Prognostic study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2017
Type:
Article
Affiliation country:
Australia
Institution/Affiliation country:
The University of Sydney/AU
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