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Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
Siqueira, Franciele Maboni; Cibulski, Samuel Paulo; Mayer, Fabiana Quoos; Driemeier, David; Pavarini, Saulo Petinatti; Vargas, Agueda Palmira Castagna de.
  • Siqueira, Franciele Maboni; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
  • Cibulski, Samuel Paulo; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
  • Mayer, Fabiana Quoos; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
  • Driemeier, David; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
  • Pavarini, Saulo Petinatti; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
  • Vargas, Agueda Palmira Castagna de; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento de Patologia Clínica Veterinária. Porto Alegre. BR
Braz. j. microbiol ; 49(1): 18-19, Jan.-Mar. 2018.
Article in English | LILACS | ID: biblio-889201
ABSTRACT
ABSTRACT Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.
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Full text: Available Index: LILACS (Americas) Main subject: Bacillus anthracis / Bacterial Vaccines / Cattle Diseases / Genome, Bacterial Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacillus anthracis / Bacterial Vaccines / Cattle Diseases / Genome, Bacterial Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR