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Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart
Almeida, Carolina Medeiros de; Lima, Rennaly de Freitas; Costa, Tereza Karla Vieira Lopes da; Sousa, Ilza Maria de Oliveira; Cabral, Elaine Cristina; Basting, Rosanna Tarkany; Torre, Adriana Della; Cavalcanti, Yuri Wanderley; Rosalen, Pedro Luiz; Duarte, Marta Cristina Teixeira; Ruiz, Ana Lúcia Tasca Gois; Foglio, Mary Ann; Godoy, Gustavo Pina; Costa, Edja Maria Melo de Brito.
  • Almeida, Carolina Medeiros de; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Lima, Rennaly de Freitas; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Costa, Tereza Karla Vieira Lopes da; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Sousa, Ilza Maria de Oliveira; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Cabral, Elaine Cristina; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Basting, Rosanna Tarkany; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Torre, Adriana Della; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Cavalcanti, Yuri Wanderley; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Rosalen, Pedro Luiz; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Duarte, Marta Cristina Teixeira; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Ruiz, Ana Lúcia Tasca Gois; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Foglio, Mary Ann; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Godoy, Gustavo Pina; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
  • Costa, Edja Maria Melo de Brito; Universidade Estadual da Paraíba - UEPB. Department of Dentistry. Campina Grande. BR
Braz. oral res. (Online) ; 32: e41, 2018. tab, graf
Article in English | LILACS | ID: biblio-889473
ABSTRACT
Abstract The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line - both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.
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Full text: Available Index: LILACS (Americas) Main subject: Candida / Plant Extracts / Biofilms / Nyctaginaceae / Cell Proliferation / Antifungal Agents Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual da Paraíba - UEPB/BR

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Full text: Available Index: LILACS (Americas) Main subject: Candida / Plant Extracts / Biofilms / Nyctaginaceae / Cell Proliferation / Antifungal Agents Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual da Paraíba - UEPB/BR