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Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform
Xiaojun, Fan; Chun, Yang; Jianhong, Liu; Chang, Zhang; Yao, Li.
  • Xiaojun, Fan; Taiyuan University of Technology. College of Chemistry and Chemical Engineering. Department of Biological and Pharmaceutical Engineering. Taiyuan. CN
  • Chun, Yang; Taiyuan University of Technology. College of Chemistry and Chemical Engineering. Department of Biological and Pharmaceutical Engineering. Taiyuan. CN
  • Jianhong, Liu; Taiyuan University of Technology. College of Chemistry and Chemical Engineering. Department of Biological and Pharmaceutical Engineering. Taiyuan. CN
  • Chang, Zhang; Taiyuan University of Technology. College of Chemistry and Chemical Engineering. Department of Biological and Pharmaceutical Engineering. Taiyuan. CN
  • Yao, Li; Taiyuan University of Technology. College of Chemistry and Chemical Engineering. Department of Biological and Pharmaceutical Engineering. Taiyuan. CN
Genet. mol. biol ; 40(1): 160-167, Jan.-Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-892366
ABSTRACT
Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI non-redundant protein database and 9,613 were annotated in the Swiss-Prot database. We mapped 4,329 unigenes onto 317 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Chitin metabolism unigenes were found in the transcriptome and the data indicated that a variety of enzymes was involved in chitin catabolic and biosynthetic pathways.


Full text: Available Index: LILACS (Americas) Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Taiyuan University of Technology/CN

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Full text: Available Index: LILACS (Americas) Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Taiyuan University of Technology/CN