Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea
Genet. mol. biol
;
40(1,supl.1): 346-359, 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-892391
ABSTRACT
Abstract Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3-) and ammonium (NH4+) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was also analyzed in C. arabica using isotopes. C. canephora N transporters follow the patterns observed for most eudicots, where each member of the AMT and NRT families has a particular role in N mobilization, and where some of these are modulated by N deficiency. Despite the prevalence of putative nitrate transporters in the Coffea genome, ammonium was the preferential inorganic N source for N-starved C. arabica roots. This data provides an important basis for fundamental and applied studies to depict molecular mechanisms involved in N uptake in coffee trees.
Full text:
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Index:
LILACS (Americas)
Type of study:
Diagnostic study
/
Risk factors
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Agronômico do Paraná/BR
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