Which is best method for instillation of topical therapy to the upper urinary tract? An in vivo porcine study to evaluate three delivery methods
Int. braz. j. urol
;
43(6): 1084-1091, Nov.-Dec. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-892917
ABSTRACT
ABSTRACT Purpose:
To compare the staining intensity of the upper urinary tract (UUT) urothelium among three UUT delivery methods in an in vivo porcine model. Materials andmethods:
A fluorescent dye solution (indigo carmine) was delivered to the UUT via three differentmethods:
antegrade perfusion, vesico-ureteral reflux via in-dwelling ureteric stent and retrograde perfusion via a 5F open-ended ureteral catheter. Twelve renal units were tested with 4 in each method. After a 2-hour delivery time, the renal-ureter units were harvested en bloc. Time from harvesting to analysis was also standardised to be 2 hours in each arm. Three urothelium samples of the same weight and size were taken from each of the 6 pre-defined points (upper pole, mid pole, lower pole, renal pelvis, mid ureter and distal ureter) and the amount of fluorescence was measured with a spectrometer.Results:
The mean fluorescence detected at all 6 predefined points of the UUT urothelium was the highest for the retrograde method. This was statistically significant with p-value less than <0.05 at all 6 points.Conclusions:
Retrograde infusion of UUT by an open ended ureteral catheter resulted in highest mean fluorescence detected at all 6 pre-defined points of the UUT urothelium compared to antegrade infusion and vesico-ureteral reflux via indwelling ureteric stents indicating retrograde method ideal for topical therapy throughout the UUT urothelium. More clinical studies are needed to demonstrate if retrograde method could lead to better clinical outcomes compared to the other two methods.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Administration, Intravesical
/
Urothelium
/
Coloring Agents
/
Indigo Carmine
Limits:
Animals
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2017
Type:
Article
Affiliation country:
Singapore
Institution/Affiliation country:
Tan Tock Seng Hospital/SG
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