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Testing the extraction of DNA from human teeth exposed to different chemical solutions / Prueba de extracción de ADN de dientes humanos expuestos a diferentes soluciones químicas
Damascena, Nicole Prata; Santos Filho, Moezio de Vasconcellos Costa; de Souza, Gustavo Reis Branco; da Silva, Luiz Antonio Ferreira; Estevam, Charles dos Santos; Franco, Ademir; Paranhos, Luiz Renato; Musse, Jamilly de Oliveira.
  • Damascena, Nicole Prata; Federal University of Sergipe. Department of Physiology. BR
  • Santos Filho, Moezio de Vasconcellos Costa; Federal University of Alagoas. Institute of Biological and Health Science. BR
  • de Souza, Gustavo Reis Branco; Federal University of Alagoas. Institute of Biological and Health Science. BR
  • da Silva, Luiz Antonio Ferreira; Federal University of Alagoas. Institute of Biological and Health Science. BR
  • Estevam, Charles dos Santos; Federal University of Sergipe. Department of Physiology. BR
  • Franco, Ademir; Federal University of Paraná. Department of Stomatology. BR
  • Paranhos, Luiz Renato; Federal University of Sergipe. Department of Dentistry. BR
  • Musse, Jamilly de Oliveira; Federal University of Feira de Santana. Department of Health. BR
Int. j. odontostomatol. (Print) ; 11(2): 173-177, June 2017. ilus
Article in English | LILACS | ID: biblio-893247
ABSTRACT
The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divided in three equal parts, which were exposed to different chemical solutions, namely hydrochloric acid (HCl) at 37 %, formaldehyde (CH2O) at 10 % and sodium hypochlorite (NaOCl) at 2.5 %. The DNA from the oral mucosa was extracted using organic method, while the dental DNA was extracted using the AFDIL method, including amplification by PCR and sequencing through capillary electrophoresis. The DNA exposed to hydrochloric acid dissolved, lacking extraction. The exposure of teeth to formaldehyde and sodium hypochlorite did not interfere in the extraction of DNA, once the amplification was visible in both experimental populations. The present outcomes demonstrated that DNA extraction may be limited under exposure to chemical solutions.
RESUMEN
El objetivo de este estudio fue evaluar la extracción de ADN dental expuesto a diferentes soluciones químicas. La muestra estuvo compuesta por 15 sujetos, de los cuales se recogieron 5 muestras de células de la mucosa oral (población de referencia) y 15 dientes (población experimental). La población experimental se dividió en tres partes iguales, que fueron expuestas a diferentes soluciones químicas, a saber, ácido clorhídrico (HCl) al 37 %, formaldehído (CH2O) al 10 % e hipoclorito de sodio (NaOCl) al 2,5 %. El ADN de la mucosa oral se extrajo utilizando el método orgánico, mientras que el ADN dental se extrajo utilizando el método AFDIL, incluyendo la amplificación por PCR y la secuenciación a través de electroforesis capilar. El ADN expuesto al ácido clorhídrico se disolvió, careciendo de extracción. La exposición de los dientes al formaldehído e hipoclorito de sodio no interfirió en la extracción de ADN, una vez que la amplificación era visible en ambas poblaciones experimentales. Los resultados actuales demostraron que la extracción de ADN puede ser limitada bajo la exposición a soluciones químicas.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: DNA Fingerprinting / Forensic Dentistry Limits: Humans Language: English Journal: Int. j. odontostomatol. (Print) Journal subject: Dentistry Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Alagoas/BR / Federal University of Feira de Santana/BR / Federal University of Paraná/BR / Federal University of Sergipe/BR

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Full text: Available Index: LILACS (Americas) Main subject: DNA Fingerprinting / Forensic Dentistry Limits: Humans Language: English Journal: Int. j. odontostomatol. (Print) Journal subject: Dentistry Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Alagoas/BR / Federal University of Feira de Santana/BR / Federal University of Paraná/BR / Federal University of Sergipe/BR