Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
J. appl. oral sci
;
25(6): 680-688, Nov.-Dec. 2017. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-893667
ABSTRACT
Abstract Objectives:
Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material andMethods:
Test materials were divided into 3 groups; Group 1(control) distilled water; Group 2 1% MC/CaCl2; Group 3 2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining.Results:
Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCI2 has decreased significantly when compared to the control (p<.05). The mRNA level of BSP has increased significantly in MTA with MC/CaCl2 compared to the control (p<.05). This study revealed higher expression of ALP and mineralization in cells exposed to MTA mixed with water and MTA mixed with MC/CaCl2 compared to the control (p<.05).Conclusions:
MC decreased the flowability of MTA and did not interrupt the physical and biological effect of MTA. It suggests that these cements may be useful as a root-end filling material.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oxides
/
Root Canal Filling Materials
/
Calcium Chloride
/
Silicates
/
Calcium Compounds
/
Aluminum Compounds
/
Methylcellulose
Limits:
Animals
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2017
Type:
Article
Affiliation country:
South Korea
Institution/Affiliation country:
Chonnam National University/KR
Similar
MEDLINE
...
LILACS
LIS