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Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner
Zhao, Qian; Hu, Yang; Deng, Shu; Yu, Pei; Chen, Bowen; Wang, Zuomin; Han, Xiaozhe.
  • Zhao, Qian; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Hu, Yang; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Deng, Shu; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Yu, Pei; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Chen, Bowen; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Wang, Zuomin; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
  • Han, Xiaozhe; Capital Medical University. Beijing ChaoYang Hospital. Department of Stomatology. Beijing. CN
J. appl. oral sci ; 26: e20170451, 2018. graf
Article in English | LILACS, BBO | ID: biblio-893699
ABSTRACT
Abstract Local administration of toll-like receptor 9 (TLR9), agonist cytidine-phosphate-guanosine oligodeoxynucleotide (CpG ODNs), and CD40 ligand (CD40L) can decrease ligature-induced periodontal inflammation and bone loss in wild type (WT) mouse.

Objective:

This study aimed to explore whether such effect is dependent on TLR9 signaling. Material and

Methods:

Purified spleen B cells isolated from WT C57BL/6J mice and TLR9 knockout (KO) mice were cultured for 48 hours under the following conditions CD40L, CpG+CD40L, CpG at low, medium and high doses. We determined B cell numbers using a hemocytometer at 24 h and 48 h. Percentages of CD1dhiCD5+ B cells were detected by flow cytometry. Interleukin-10 (IL-10) mRNA expression and protein secretion were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and by ELISA, respectively. The silk ligature was tied around the maxillary second molars for 14 days, during which the CpG+CD40L mixture or PBS was injected into palatal gingiva on days 3, 6, and 9.

Results:

For both WT and TLR9 KO mice, CpG significantly induced B cell proliferation, increased IL-10 mRNA expression and protein secretion of IL-10 but reduced CD1dhiCD5+ B cells population; local injection of CpG+CD40L mixture significantly decreased alveolar bone loss and the number of TRAP-positive cells adjacent to the alveolar bone surface, and significantly increased the gingival mRNA expression of IL-10 and decreased RANKL and IFN-γ mRNA expression.

Conclusions:

These results indicated that CpG plus CD40L decreased periodontal inflammation and alveolar bone loss in a TLR9-independent manner in ligature-induced experimental periodontitis.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oligodeoxyribonucleotides / Periodontitis / Alveolar Bone Loss / CD40 Ligand / Cytidine / Toll-Like Receptor 9 / Guanine Nucleotides Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Capital Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Oligodeoxyribonucleotides / Periodontitis / Alveolar Bone Loss / CD40 Ligand / Cytidine / Toll-Like Receptor 9 / Guanine Nucleotides Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Capital Medical University/CN