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In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics
Souza, Claudemir; Zanchin, Nilson IT; Krieger, Marco A; Ludwig, Adriana.
  • Souza, Claudemir; Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba. BR
  • Zanchin, Nilson IT; Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba. BR
  • Krieger, Marco A; Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba. BR
  • Ludwig, Adriana; Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba. BR
Mem. Inst. Oswaldo Cruz ; 112(10): 655-663, Oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-894834
Responsible library: BR1.1
ABSTRACT
BACKGROUND The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes. OBJECTIVES To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. METHODS We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. FINDINGS The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. MAIN CONCLUSIONS We have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
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Full text: Available Index: LILACS (Americas) Main subject: Peptide Synthases / Respiratory Syncytial Viruses / Genetic Variation / Viral Proteins / Genotype Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2017 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR

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Full text: Available Index: LILACS (Americas) Main subject: Peptide Synthases / Respiratory Syncytial Viruses / Genetic Variation / Viral Proteins / Genotype Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2017 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR