Standardization and evaluation of a duplex real-time quantitative PCR for the detection of Leishmania infantum DNA: a sample quality control approach
Rev. Soc. Bras. Med. Trop
;
50(3): 350-357, May-June 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-896966
ABSTRACT
Abstract INTRODUCTION:
Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol developed for the simultaneous detection of Leishmania infantum DNA and sample quality control.METHODS:
After preliminary tests with the newly designed TaqMan® probes for the two targets ( L. infantum and glyceraldehyde 3-phosphate dehydrogenase (G3PD) gene), the duplex qPCR protocol was optimized. For the evaluation of the standardized protocol, human blood samples were tested (n=68) and the results were compared to those obtained by reference diagnostic techniques. Statistical analyses included percentage agreement and the Kappa ( k ) coefficient.RESULTS:
The detection limit of L. infantum DNA reached 2x10 2 fg (corresponding to ~1 parasite) per µL of blood (ε 93.9%). The percentage agreement obtained between the duplex VL qPCR and the reference techniques was individually obtained as follows molecular 88.3% ( k =0.666; 95% CI 0.437-0.894, good), and serological 81.7% ( k =0.411; 95% CI 0.125-0.697, moderate). Between the reference techniques, the percentage agreement was 86.7% ( k =0.586; 95% CI 0.332-0.840, moderate).CONCLUSIONS:
The new duplex VL qPCR protocol indicated good potential for the accurate, fast, and reliable detection of L. infantum DNA, when applied as a complement to the classical diagnostic tools already available, especially in health or research reference centers.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Quality Control
/
DNA, Protozoan
/
Leishmania infantum
/
Real-Time Polymerase Chain Reaction
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
/
Evaluation studies
/
Practice guideline
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2017
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Pesquisas Aggeu Magalhães/BR
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