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Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI. 7 and GV. 2 sapoviruses in Brazil
Costa, Larissa Cristina Prado das Neves; Siqueira, Jones Anderson Monteiro; Portal, Thayara Morais; Sousa Júnior, Edivaldo Costa; Linhares, Alexandre da Costa; Gabbay, Yvone Benchimol; Resque, Hugo Reis.
  • Costa, Larissa Cristina Prado das Neves; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Siqueira, Jones Anderson Monteiro; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Portal, Thayara Morais; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Sousa Júnior, Edivaldo Costa; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Linhares, Alexandre da Costa; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Gabbay, Yvone Benchimol; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
  • Resque, Hugo Reis; Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém. BR
Rev. Soc. Bras. Med. Trop ; 50(5): 621-628, Sept.-Oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-897017
ABSTRACT
Abstract

INTRODUCTION:

Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992.

METHODS:

HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing.

RESULTS:

Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected.

CONCLUSIONS:

The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Brazil / Adenoviruses, Human / Caliciviridae Infections / Sapovirus / Gastroenteritis / Genotype Type of study: Diagnostic study / Observational study / Prevalence study / Risk factors Limits: Child, preschool / Female / Humans / Infant / Male / Infant, Newborn Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade do Estado do Pará/BR

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Full text: Available Index: LILACS (Americas) Main subject: Brazil / Adenoviruses, Human / Caliciviridae Infections / Sapovirus / Gastroenteritis / Genotype Type of study: Diagnostic study / Observational study / Prevalence study / Risk factors Limits: Child, preschool / Female / Humans / Infant / Male / Infant, Newborn Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade do Estado do Pará/BR