Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
Rev. Soc. Bras. Med. Trop
;
51(2): 168-173, Mar.-Apr. 2018. graf
Article
in English
| LILACS
| ID: biblio-897058
ABSTRACT
Abstract INTRODUCTION:
Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.METHODS:
In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.CONCLUSIONS:
The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Leukocytes, Mononuclear
/
Dengue
/
Dengue Virus
/
Antibodies, Viral
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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