Expresión de O6-metilguanina-ADN-metiltransferasa (MGMT) en pacientes chilenos con glioblastoma multiforme / O6-methylguanine-DNA-methyltransferase (MGMT) expression in patients with glioblastoma multiforme
Rev. méd. Chile
;
146(1): 7-14, ene. 2018. tab, graf
Article
in Spanish
| LILACS
| ID: biblio-902616
ABSTRACT
Background:
Patients with Glioblastoma multiforme (GBM) have a five years survival of less than 5%, but the response to chemotherapy with alkylating agents can vary depending on the methylation status of O6-methylguanine-DNA-methyltransferase (MGMT). Genetic testing has limitations for routine use, while immunohistochemistry (IHC) offers a fast and affordable technique but with heterogeneous results in the literature.Aim:
To evaluate MGMT expression by IHC in tumor tissue of Chilean patients with GBM. Material andMethods:
Tumor samples of 29 patients with a pathological diagnosis of GBM were studied. We performed IHC staining and manual analysis of positive and negative cells for MGMT expression. A cut-off of at least 10% of cells expressing MGMT was used. Demographic and clinical features of patients were obtained from clinical records.Results:
The median number of cells counted per case was 692 (interquartile range [IQR] 492-928). Fifteen cases (52%) were positive for MGMT expression. Median overall survival was 5.3 months (IQR 3.4-12-8). The effect of MGMT expression on the therapeutic response was not studied since only 3 patients received chemotherapy.Conclusions:
Our results are similar to international reports, but we were not able to determine the association between MGMT expression and therapeutic response.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Brain Neoplasms
/
Biomarkers, Tumor
/
Glioblastoma
/
O(6)-Methylguanine-DNA Methyltransferase
Type of study:
Practice guideline
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2018
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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