Prevalence of HPV and EBV infection andtheir relationship with the p53 and PCNA expression in oral carcinoma patients
J. oral res. (Impresa)
;
6(4): 86-91, Apr. 2017. tab
Article
in English
| LILACS
| ID: biblio-907721
ABSTRACT
Introduction:
Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions.Objective:
To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma.Methodology:
Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining.Results:
The mean age of the patients was 60.55 +/- 13.94 years, and 52 percent of these were female. Of the patients, 59 percent were tobacco users and 63 percent were alcohol consumers. HPV was detected in 70 percent of the patients with the predominance of genotype 16 (60 percent). As for EBV infection, it was observed in 59 percent of cases. p53 and PCNA immunopositivity corresponded to 44 percent and 59 percent, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48 percent of the cases presented infection by both viruses.Conclusion:
HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
Type of study:
Prevalence study
/
Risk factors
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
J. oral res. (Impresa)
Journal subject:
Dentistry
Year:
2017
Type:
Article
/
Project document
Affiliation country:
Venezuela
Institution/Affiliation country:
Hospital Oncológico Padre Machado/VE
/
Instituto de Investigaciones Odontológicas Raúl Vincentelli/VE
/
Instituto de Oncología y Hematología-MPPS/VE
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