Cirrhosis in rats does not resolve in the long-term after induction by thioacetamide model
Braz. j. morphol. sci
;
31(1): 33-41, 1/3/2014. ilus, tab
Article
in English
| LILACS
| ID: biblio-912339
ABSTRACT
Introductions Hepatic cirrhosis is a final common pathway of all chronic liver diseases, characterized by deposit of fibrillar collagen and liver failure. Materials and Methods:
In this experiment, hepatic cirrhosis was induced in 15 female Wistar rats by a 14-week period, with thioacetamide solution in a 200 mg/kg dosage, via intraperitoneal. Animals were submitted to liver biopsy, and euthanized after a 80-day post-induction period. Serum biochemical analysis was performed, in addition to histopathology by H.E., Picrosirius, Alcian Blue and P.A.S. stainings, following analysis of histological activity index and staging of fibrosis. Morphometric analysis of collagen on Picrosirius slides was also performed.Results:
Mortality during experimental period was low (13.33%), and after 80-day period, liver function improved, cellular changes did not altered, and deposition of acidic mucopolysaccharides and glycogen were increased. Liver histological activity did not change significantly (7.25 ± 1.30 to 6.41 ± 1.32), but staging of fibrosis was altered (3.91 ± 0.76 to 4.70 ± 1.11). Interlobular collagen showed a significant decrease (5.14 ± 2.00 to 4.00 ± 1.20), while intralobular collagen was increased (0.23 ± 0.06 to 0.36 ± 0.08).Conclusions:
These findings characterize thioacetamide as a safe experimental model for induction cirrhosis, which may be used for future therapy studies.(AU)
Full text:
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Index:
LILACS (Americas)
Main subject:
Thioacetamide
/
Collagen
/
Disease Models, Animal
/
Liver Cirrhosis
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Braz. j. morphol. sci
Journal subject:
Anatomy
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of São Paulo/BR
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