Profile of exoantigens from clinical isolates of Paracoccidioides lutzii IN Mato Grosso, Brazil
Rev. patol. trop
;
45(3): 265-272, set. 2016. tab, ilus
Article
in English
| LILACS
| ID: biblio-913231
ABSTRACT
The objective of this study was to determine the immunochemical profile of three exoantigen preparations obtained from clinical samples of Paracoccidioides lutzii from the state of Mato Grosso, Brazil. The exoantigens were prepared according to a protocol standardized by Camargo et al. and their immunochemical profiles were analyzed using SDS-PAGE. The observed protein bands exhibited molecular weights of 20, 30, and 130 kDa. The 130 kDa glycoprotein was detected in all three preparations. The detection of this high-molecular-weight protein suggests a distinct immunochemical profile. These differences may help to explain the false negative reactions obtained when using the antigen from P. brasiliensis reference strain B-339. This information is essential for increasing the specificity of serological tests used to monitor the prognosis of patients with paracoccidioidomycosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paracoccidioides
Type of study:
Practice guideline
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. patol. trop
Journal subject:
Tropical Medicine
/
Pathology
Year:
2016
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Assistant Secretary of Policy and Health Care Department of Health, State Department of Health, SES/BR
/
Department of Epidemiological Surveillance Coordination, Central Laboratory (LACEN-MT)/BR
/
Mycology Laboratory, Federal University of São Paulo (UNIFESP)/BR
/
Post-Graduate Program in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso/BR
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