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Reliability of different methods of diagnosing intestinal parasites in horses
Macedo, Lucia Oliveira de; Santos, Marcos Antônio Bezerra; Alves, Leucio Câmara; Ramos, Rafael Antonio Nascimento; Carvalho, Gílcia Aparecida de.
  • Macedo, Lucia Oliveira de; Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE). Garanhuns. BR
  • Santos, Marcos Antônio Bezerra; Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE). Garanhuns. BR
  • Alves, Leucio Câmara; Departamento de Medicina Veterinária, UFRPE. Recife. BR
  • Ramos, Rafael Antonio Nascimento; Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE). Garanhuns. BR
  • Carvalho, Gílcia Aparecida de; Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE). Garanhuns. BR
Rev. patol. trop ; 46(4): 331-338, dez. 2017. tab
Article in English | LILACS | ID: biblio-913750
ABSTRACT
Parasitism by intestinal nematodes may cause serious injuries to equines affecting their performance, given that the parasites compete for food and cause irritation, internal bleeding and anaemia. The diagnostic methods currently available are not efficient in detecting all the species of parasites simultaneously, hampering reliable diagnosis. Therefore, the purpose of this study was to evaluate four diagnostic methods for detecting equine intestinal parasites in the southern hinterland of Pernambuco, Brazil. Fecal samples (n = 87) were collected and examined through flotation based methods (Willis-Mollay, modified centrifugal flotation and EPG/OPG) and sedimentation (Hoffman). Of the total number of samples examined, 77.0% (67/87) were positive by modified centrifugal flotation; 44.8% (39/87) were positive by the Willis-Mollay method; 34.5% (30/87) by the Hoffman method and 28.7% (25/87) by the EPG/ OPG method. The Kappa index indicated moderate concordance between the Willis-Mollay and modified centrifugal flotation techniques (K= 0.477); Willis-Mollay and EPG/OPG (K= 0.466); EPG/OPG and Hoffman (K= 0.425). In conclusion, modified centrifugal flotation presented high sensitivity for detection of parasites of the Strongylida order and Parascaris spp. It may, therefore, be used in association with the Willis-Mollay technique as a safe and accurate method of diagnosis.
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Full text: Available Index: LILACS (Americas) Main subject: Strongylida Type of study: Diagnostic study Language: English Journal: Rev. patol. trop Journal subject: Tropical Medicine / Pathology Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Departamento de Medicina Veterinária, UFRPE/BR / Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE)/BR

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Full text: Available Index: LILACS (Americas) Main subject: Strongylida Type of study: Diagnostic study Language: English Journal: Rev. patol. trop Journal subject: Tropical Medicine / Pathology Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Departamento de Medicina Veterinária, UFRPE/BR / Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE)/BR