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Estudio e identificación de microRNA en exosomas urinarios en hipertensión arterial mineralocorticoide / Study and identification of microRNA in urinary exosomes in mineralocorticoid arterial hypertension
Tapia-Castillo, Alejandra; Valdivia P, Carolina; Campino J, Carmen; Allende, Fidel; Solari, Sandra; Ortiz, David; Barros, Eric; Vecchiola, Andrea; Baudrand, Rene; Fardella, Carlos; Carvajal M, Cristian.
  • Tapia-Castillo, Alejandra; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Valdivia P, Carolina; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Campino J, Carmen; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Allende, Fidel; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Laboratorios Clínicos. CL
  • Solari, Sandra; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Laboratorios Clínicos. CL
  • Ortiz, David; Pontificia Universidad Católica de Chile. Facultad de Ciencias de la Salud. Departamento de Endocrinología. CL
  • Barros, Eric; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Vecchiola, Andrea; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Baudrand, Rene; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Fardella, Carlos; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
  • Carvajal M, Cristian; Pontificia Universidad Católica de Chile. Facultad de Medicina. Departamento de Endocrinología. CL
Rev. chil. endocrinol. diabetes ; 11(2): 54-61, abr. 2018. ilus, tab
Article in Spanish | LILACS | ID: biblio-914726
ABSTRACT
About 15% of the essential hypertensive patients would have a low activity of the 11ßHSD2 enzyme, which inactivates cortisol (F) to cortisone (E). Gene expression can be negatively regulated by miRNA. Urinary exosomes and their specific content (miRNA/proteins) represent a valuable tool as a biomarker for the diagnosis and prognosis of the disease.

Aim:

To evaluate the expression of miRNA specific for 11ßHSD2 in samples of urinary exosomes and to determine its association with biochemical variables associated with mineralocorticoid metabolism. Subjects and

Methods:

Cross-sectional study in subjects between 10-60 years. They were classified into subjects with high F/E (> p75) and low cortisone (< p25) and control subjects. The urinary exosomes were isolated with the Invitrogen kit. Bioinformatic analysis was performed with Mir Walk to identify specific miRNAs of HSD11B2. The expression of miRNA was evaluated by qRT PCR. The comparisons were made with the Mann-Whitney test.

Results:

7.1% of the subjects are suggestive of a partial deficiency of 11ßHSD2 (NC-AME). The expression of miR-488 was higher in NC-AME than in controls (5839 ± 1719 vs 3,437 ± 2,581; p = 0.01). We found positive associations between mir-615 and ARP; miR-488 and the sodium/potassium ratio; miR-1205 with age and urinary sodium excretion; miR-494 with age, activity MMP9 and NGAL.

Conclusion:

We identified high expression of miR488 in NC-AME subjects and associations of miRNAs with biochemical variables associated with mineralocorticoid metabolism. Thus, exosomes and their miRNA content could be potential regulators and biomarkers of 11ßHSD2 activity.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Receptors, Mineralocorticoid / MicroRNAs / 11-beta-Hydroxysteroid Dehydrogenase Type 2 / Exosomes / Hypertension Type of study: Diagnostic study / Observational study / Prevalence study / Prognostic study / Risk factors Limits: Adolescent / Adult / Child / Female / Humans / Male Language: Spanish Journal: Rev. chil. endocrinol. diabetes Journal subject: Endocrinology Year: 2018 Type: Article / Project document Affiliation country: Chile Institution/Affiliation country: Pontificia Universidad Católica de Chile/CL

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Full text: Available Index: LILACS (Americas) Main subject: Receptors, Mineralocorticoid / MicroRNAs / 11-beta-Hydroxysteroid Dehydrogenase Type 2 / Exosomes / Hypertension Type of study: Diagnostic study / Observational study / Prevalence study / Prognostic study / Risk factors Limits: Adolescent / Adult / Child / Female / Humans / Male Language: Spanish Journal: Rev. chil. endocrinol. diabetes Journal subject: Endocrinology Year: 2018 Type: Article / Project document Affiliation country: Chile Institution/Affiliation country: Pontificia Universidad Católica de Chile/CL