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The m-rna, expression of serca2 and ncx1 in the process of pharmacological cell protection in experimental acute pancreatitis induced by taurocholate / Expressão do rna-m, da serca2 e do ncx1 no processo de proteção celular farmacológica na pancreatite aguda experimental induzida por taurocolato
Vasques, Enio Rodrigues; Cunha, José Eduardo Monteiro; Kubrusly, Marcia Saldanha; Coelho, Ana Maria; Sanpietri, Sandra N; Nader, Helena B; Tersariol, Ivarne L S; Lima, Marcelo A; Chaib, Eleazar; D'Albuquerque, Luiz Augusto Carneiro.
  • Vasques, Enio Rodrigues; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • Cunha, José Eduardo Monteiro; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • Kubrusly, Marcia Saldanha; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • Coelho, Ana Maria; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • Sanpietri, Sandra N; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • Nader, Helena B; Federal University of São Paulo. Pharmacology Department. São Paulo. BR
  • Tersariol, Ivarne L S; Federal University of São Paulo. Pharmacology Department. São Paulo. BR
  • Lima, Marcelo A; Federal University of São Paulo. Pharmacology Department. São Paulo. BR
  • Chaib, Eleazar; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
  • D'Albuquerque, Luiz Augusto Carneiro; University of São Paulo. Medical School. Gastroenterology Department LIM 37. BR
ABCD (São Paulo, Impr.) ; 31(1): e1352, 2018. tab
Article in English | LILACS | ID: biblio-949203
ABSTRACT
ABSTRACT

Background:

Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP.

Aim:

To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD.

Methods:

Wistar rats were divided in groups 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR).

Results:

Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2.

Conclusions:

The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.
RESUMO
RESUMO Racional A lesão celular da pancreatite aguda (PA) envolve sobrecarga de cálcio, regulada pela atividade da Cálcio ATPase de membrana (PMCA), Cálcio ATPase do Retículo (SERCA2) e pelo Trocador Sódio Cálcio (NCX1). A melatonina (antioxidante) e o Dissacarídeo Trissulfatado (acelerador do NCX1) poderiam reduzir a lesão celular na PA.

Objetivo:

Avaliar a expressão do RNAm da SERCA2 e NCX1 em modelo animal de pancreatite aguda tratados com melatonina e/ou dissacarídeo trissulfatado (DT).

Método:

Ratos Wistar foram divididos em grupos 1) sem pancreatite aguda; 2) com pancreatite aguda por taurocolato; 3) PA e Melatonina; 4) PA e DT; 5) PA e Melatonina com DT. Amostras de tecido foram colhidas para detecção dos níveis de RNAm da SERCA2 e NCX1 por PCR.

Resultados:

Houve aumento da expressão do RNAm da SERCA2 no grupo com PA tratados com Melatonina, porém sem aumento de expressão do NCX1. O DT não afetou os níveis de SERCA2 e NCX1. O tratamento conjunto com Melatonina e DT diminuiu a expressão da SERCA2.

Conclusões:

O efeito da Melatonina é restrito ao aumento da expressão da SERCA2. O DT não tem ação na expressão gênica, porém sua ação na aceleração do trocador na retirada do cálcio pode explicar a menor expressão da SERCA2 quando associado à Melatonina, pela ação conjunta de drogas com mecanismos diferentes e possivelmente complementares.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Pancreatitis / RNA, Messenger / Sodium-Calcium Exchanger / Cytoprotection / Sarcoplasmic Reticulum Calcium-Transporting ATPases Type of study: Prognostic study Limits: Animals Language: English Journal: ABCD (São Paulo, Impr.) Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of São Paulo/BR / University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Pancreatitis / RNA, Messenger / Sodium-Calcium Exchanger / Cytoprotection / Sarcoplasmic Reticulum Calcium-Transporting ATPases Type of study: Prognostic study Limits: Animals Language: English Journal: ABCD (São Paulo, Impr.) Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of São Paulo/BR / University of São Paulo/BR