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Effects of remote ischemic preconditioning and topical hypothermia in renal ischemia-reperfusion injury in rats
Motta, Guilherme Lang; Souza, Pablo Cambeses; Santos, Emanuel Burck dos; Bona, Silvia Regina; Schaefer, Pedro Guilherme; Lima, Caetano Araújo Torres; Marroni, Norma Anair Possa; Corso, Carlos Otávio.
  • Motta, Guilherme Lang; Universidade Federal do Rio Grande do Sul. Postgraduate Program in Medicine: Surgical Sciences. Porto Alegre. BR
  • Souza, Pablo Cambeses; UFRGS. Postgraduate Program in Medicine: Surgical Sciences. Porto Alegre. BR
  • Santos, Emanuel Burck dos; Hospital de Clínicas de Porto Alegre. Department of Urology. Porto Alegre. BR
  • Bona, Silvia Regina; HCPA. Laboratory of Experimental Gastroenterology and Hepatology. Porto Alegre. BR
  • Schaefer, Pedro Guilherme; HCPA. Department of Pathology. Porto Alegre. BR
  • Lima, Caetano Araújo Torres; UFRGS. School of Medicine. Porto Alegre. BR
  • Marroni, Norma Anair Possa; HCPA. Laboratory of Experimental Gastroenterology and Hepatology. Porto Alegre. BR
  • Corso, Carlos Otávio; UFRGS. Department of Surgery. Porto Alegre. BR
Acta cir. bras ; 33(5): 396-407, May 2018. tab, graf
Article in English | LILACS | ID: biblio-949345
ABSTRACT
Abstract

Purpose:

To evaluate whether combining hypothermia and remote ischemic preconditioning (RIPC) results in protection from ischemia-reperfusion (IR).

Methods:

Thirty-two Wistar rats underwent right nephrectomy and were randomly assigned to four experimental protocols on the left kidney warm ischemia (group 1), cold ischemia (group 2), RIPC followed by warm ischemia (group 3), and RIPC followed by cold ischemia (group 4). After 240 minutes of reperfusion, histological changes in the left kidney, as well as lipid peroxidation and antioxidant enzyme activity, were analyzed. The right kidney was used as the control. Serum creatinine was collected before and after the procedures.

Results:

RIPC combined with hypothermia during IR experiments revealed no differences on interventional groups regarding histological changes (p=0.722). Oxidative stress showed no significant variations among the groups. Lower serum creatinine at the end of the procedure was seen in animals exposed to hypothermia (p<0.001).

Conclusions:

Combination of RIPC and local hypothermia provides no renal protection in IR injury. Hypothermia preserves renal function during ischemic events. Furthermore, RIPC followed by warm IR did not show benefits compared to warm IR alone or controls in our experimental protocol.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Oxidative Stress / Ischemic Preconditioning / Hypothermia, Induced / Kidney Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: HCPA/BR / Hospital de Clínicas de Porto Alegre/BR / UFRGS/BR / Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Oxidative Stress / Ischemic Preconditioning / Hypothermia, Induced / Kidney Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: HCPA/BR / Hospital de Clínicas de Porto Alegre/BR / UFRGS/BR / Universidade Federal do Rio Grande do Sul/BR