The role of vitamin C in the gene expression of oxidative stress markers in fibroblasts from burn patients
Acta cir. bras
;
33(8): 703-712, Aug. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-949375
ABSTRACT
Abstract Purpose:
To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients.Methods:
Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups.Results:
The comparison revealed 10 upregulated genes as follows arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase (DHCR24), dual oxidase 1 (DUOX1), glutathione peroxidase 2 (GPX2), glutathione peroxidase 5 (GPX5), microsomal glutathione S-transferase 3 (MGST3), peroxiredoxin 4 (PRDX4), phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (P-REX1), prostaglandin-endoperoxide synthase 1 (PTGS1), and ring finger protein 7 (RNF7).Conclusion:
Cultured fibroblasts obtained from burn patients and treated with vitamin C resulted in 10 differentially expressed genes, all overexpressed, with DUOX1, GPX5, GPX2 and PTGS1 being of most interest.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ascorbic Acid
/
Burns
/
Gene Expression
/
Oxidative Stress
/
Fibroblasts
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UNIFESP/BR
/
Universidade Federal de São Paulo/BR
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