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Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
Schiavoni, Vagner Sarraipo; Silva, Jairo Pinheiro da; Lizarte Neto, Fermino Sanches; Assis, Múcio Luiz Cirino de; Tazima, Maria de Fátima Galli Sorita; Carvalho, Camila Albuquerque Melo de; Tirapelli, Daniela Pretti da Cunha; Carlotti Jr, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luis Fernando.
  • Schiavoni, Vagner Sarraipo; Universidade de São Paulo. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Silva, Jairo Pinheiro da; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Lizarte Neto, Fermino Sanches; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Assis, Múcio Luiz Cirino de; Medical School. Department of Surgery and Anatomy. Postgraduate Program in Clinical Surgery. Ribeirao Preto. BR
  • Tazima, Maria de Fátima Galli Sorita; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Carvalho, Camila Albuquerque Melo de; Universidade Federal de Alagoas. Institute of Biological and Health Sciences. Maceio. BR
  • Tirapelli, Daniela Pretti da Cunha; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Carlotti Jr, Carlos Gilberto; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Colli, Benedicto Oscar; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Tirapelli, Luis Fernando; USP. Medical School. Department of Surgery and Anatomy. Ribeirao Preto. BR
Acta cir. bras ; 33(8): 652-663, Aug. 2018. graf
Article in English | LILACS | ID: biblio-949377
Responsible library: BR1.1
ABSTRACT
Abstract

Purpose:

To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model.

Methods:

Forty adult Wistar rats were used, subdivided into 5 experimental groups control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A) animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP).

Results:

Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins.

Conclusion:

The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ischemic Attack, Transient / Alcoholism / Inhibitor of Apoptosis Proteins / Caspase 3 Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Medical School/BR / USP/BR / Universidade Federal de Alagoas/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ischemic Attack, Transient / Alcoholism / Inhibitor of Apoptosis Proteins / Caspase 3 Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Medical School/BR / USP/BR / Universidade Federal de Alagoas/BR / Universidade de São Paulo/BR