Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
Biol. Res
; 48: 1-8, 2015. graf
Article
in En
| LILACS
| ID: biblio-950773
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.RESULTS:
This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as IL-1ß and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-kBpathway.CONCLUSIONS:
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.Key words
Full text:
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Index:
LILACS
Main subject:
Aging
/
Drugs, Chinese Herbal
/
Plant Extracts
/
Dermis
/
Matrix Metalloproteinase 1
/
Fibroblasts
Limits:
Humans
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2015
Type:
Article