Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Biol. Res
;
48: 1-7, 2015. ilus, graf
Article
in English
| LILACS
| ID: biblio-950782
ABSTRACT
BACKGROUND:
Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells.RESULTS:
In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells.CONCLUSIONS:
All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cell Cycle
/
Carcinoma, Hepatocellular
/
Lentivirus
/
RNA Interference
/
Cell Proliferation
/
Ubiquitin-Specific Proteases
/
Liver Neoplasms
/
Neoplasm Proteins
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Second Military Medical University/CN
/
Shanghai Municipal Center for Disease Control and Prevention/CN
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