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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Pan, Zeya; Pan, Hao; Zhang, Jin; Yang, Yun; Liu, Hui; Yang, Yuan; Huang, Gang; Ni, Junsheng; Huang, Jian; Zhou, Weiping.
  • Pan, Zeya; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Pan, Hao; Shanghai Municipal Center for Disease Control and Prevention. Department of Infectious Disease Control and Prevention. Shanghai. CN
  • Zhang, Jin; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Yang, Yun; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Liu, Hui; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Yang, Yuan; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Huang, Gang; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Ni, Junsheng; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Huang, Jian; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
  • Zhou, Weiping; Second Military Medical University. Eastern Hepatobiliary Surgery Hospital. The Third Department of Hepatic Surgery. Shanghai. CN
Biol. Res ; 48: 1-7, 2015. ilus, graf
Article in English | LILACS | ID: biblio-950782
ABSTRACT

BACKGROUND:

Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells.

RESULTS:

In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells.

CONCLUSIONS:

All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC.
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Full text: Available Index: LILACS (Americas) Main subject: Cell Cycle / Carcinoma, Hepatocellular / Lentivirus / RNA Interference / Cell Proliferation / Ubiquitin-Specific Proteases / Liver Neoplasms / Neoplasm Proteins Limits: Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2015 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Second Military Medical University/CN / Shanghai Municipal Center for Disease Control and Prevention/CN

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Full text: Available Index: LILACS (Americas) Main subject: Cell Cycle / Carcinoma, Hepatocellular / Lentivirus / RNA Interference / Cell Proliferation / Ubiquitin-Specific Proteases / Liver Neoplasms / Neoplasm Proteins Limits: Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2015 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Second Military Medical University/CN / Shanghai Municipal Center for Disease Control and Prevention/CN