Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
Biol. Res
;
48: 1-7, 2015. ilus, graf, tab
Article
in English
| LILACS
| ID: biblio-950804
ABSTRACT
BACKGROUND:
We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells).RESULTS:
MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells.CONCLUSION:
Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Benzopyrans
/
Plant Extracts
/
Apoptosis
/
Cell Proliferation
/
Cell Cycle Checkpoints
/
Fabaceae
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2015
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Macau University of Science and Technology/CN
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