Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
Biol. Res
;
48: 1-8, 2015. ilus, graf, tab
Article
in English
| LILACS
| ID: biblio-950806
ABSTRACT
BACKGROUND:
Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogen-esis in retinal pigment epithelium (RPE).RESULTS:
We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium-derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased.CONCLUSIONS:
RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Serpins
/
Vascular Endothelial Growth Factor A
/
Eye Proteins
/
Retinal Pigment Epithelium
/
Hypothermia
/
Nerve Growth Factors
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2015
Type:
Article
Affiliation country:
Japan
Institution/Affiliation country:
Aichi Medical University/JP
/
Aichi Prefectural University/JP
/
University of Tsukuba/JP
Similar
MEDLINE
...
LILACS
LIS