Suppression of miR-181 a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression
Biol. Res
;
48: 1-7, 2015. graf
Article
in English
| LILACS
| ID: biblio-950809
ABSTRACT
BACKGROUND:
Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of HKII could improve the survival of mesenchymal stem cells (MSCs) exposed to H2O2. The expression of HKII in MSCs exposed to H2O2 was evaluated, and HKII-targeting miRNA was screened based on miRNA-target prediction databases. The effect of H2O2 on the expression of the selected HKII-targeting miRNA was examined and the effect of modulation of the selected HKII-targeting miRNA using anti-miRNA on H2O2-induced apoptosis of MSC was evaluated.RESULTS:
H2O2 (600 µM) induced cell death of MSCs and decreased mitochondrial HKII expression. We have identified miR-181a as a HKII-targeting miRNA and H2O2 increased the expression of miR-181a in MSCs. Delivery of anti-miR-181a, which neutralizes endogenous miR-181a, significantly attenuated H2O2-induced decrease of HKII expression and disruption of mitochondrial membrane potential, improving the survival of MSCs exposed to H2O2.CONCLUSIONS:
These findings suggest that H2O2-induced up-regulation of miR-181a contributes to the cell death of MSCs by down-regulating HKII. Neutralizing miR-181a can be an effective way to prime MSCs for transplantation into ischemic tissues.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Apoptosis
/
MicroRNAs
/
Mesenchymal Stem Cells
/
Glioma
/
Hexokinase
/
Hydrogen Peroxide
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2015
Type:
Article
Affiliation country:
South Korea
Institution/Affiliation country:
Catholic Kwandong University/KR
/
Yonsei University/KR
/
Yonsei University, Seoul/KR
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