Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
Biol. Res
;
48: 1-6, 2015. ilus, tab
Article
in English
| LILACS
| ID: biblio-950814
ABSTRACT
BACKGROUND:
DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz. DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world.RESULTS:
Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively.CONCLUSIONS:
Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Colorectal Neoplasms
/
Genes, Tumor Suppressor
/
DNA Methylation
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Amazon State University/BR
/
Carlos Chagas Institute/BR
/
FIOCRUZ/BR
/
Federal University of Amazonas/BR
/
Federal University of Rio de Janeiro/BR
/
Oncology Control Center Foundation of Amazon State/BR
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