Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
Biol. Res
;
49: 1-9, 2016. ilus, graf
Article
in English
| LILACS
| ID: biblio-950863
ABSTRACT
BACKGROUND:
Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin.RESULTS:
GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4-CD8-) and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+) thymocytes.CONCLUSIONS:
The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Thymus Gland
/
Growth Hormone
/
Laminin
/
Epithelial Cells
/
Thymocytes
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Child
/
Child, preschool
/
Humans
/
Infant
/
Infant, Newborn
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2016
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Alagoas/BR
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